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. 2014 Oct 9;9(10):e109630. doi: 10.1371/journal.pone.0109630

Figure 6. ROS generation and DNA damage accumulation in carnosol-treated MDA-MB-231 cells.

Figure 6

(A) Carnosol induces ROS generation in MDA-MB-231 cells. Cells were treated with indicated concentrations of carnosol for 24 h and ROS production was analyzed, as described in Material and Methods, using DCFDA fluorescence stain. (B) time-course measurement of ROS generation in carnosol-treated MDA-MB-231 cells. Cells were treated with 100 µM carnosol and ROS generation was examined at different time-point (1, 3 and 6 h). (C–D) Concentration and time-dependent accumulation of γH2AX, marker of DNA damage, in carnosol-treated cells. DNA damage was evaluated by measuring, by Western blotting, the level γH2AX accumulation using anti-phospho-H2AX (ser 139) antibody.