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. 2014 Oct 9;9(10):e109595. doi: 10.1371/journal.pone.0109595

Figure 3. Pharmacological inhibition of p38 MAPK pathways prevented the regulation of aggrecan, ADAMTS-4 and ADAMTS-5 by leptin.

Figure 3

(A) The protein amouts of phosphorylated forms of p38 (P- p38 were detected with western blotting analysis. GAPDH was also detected for a loading control. (B) NP cells were treated with vehicle (contorl) (-), 10 ng/ml leptin (+), 10 µM SB203580 for 48 h. The amouts of aggrecan, ADAMTS-4 and ADAMTS-5 protein were detected with western blotting analysis using GAPDH as an internal control. (C) Aggrecan, ADAMTS-4 and ADAMTS-5 mRNA expression were detected with Real-time RT-PCR analysis using GAPDH as an internal control. Error bars represent standard deviration. The medium was changed every day.***p<0.001.