Table 2.
Methods for the detection of immunohistochemical expression of the tested markers
| Company | Antigen | Blocking | 1.AK | 2.AK | |
|---|---|---|---|---|---|
| retrieval | 30′RT | ||||
| AREG | R&D Systems | 0,1 M | 5% swine | 1:75, | LSAB + System-HRP |
| AF262 | EDTA buffer pH8 | serum | over night | K 0690 | |
| EREG | R&D Systems | - | 5% swine | 1:100 | LSAB + System-HRP |
| AF1195 | serum | over night | K 0690 | ||
| VEGF-C | Sant Cruz | - | FFP | 1:50 | Dako Real™EnVision™Detection System |
| sc-9047 | over night | K 5007 | |||
| VEGF-D | Sant Cruz | 0.01 M | FFP | 1:50 | Dako Real™EnVision™Detection System |
| sc-13085 | Citrat buffer | 2 h RT | K 5007 | ||
| VEGFR-3 | Sant Cruz | 0.01 M | FFP | 1:100 | LSAB + System-HRP |
| sc-321 | Citrat buffer | 2 h RT | K 0690 | ||
| Hif-1 α | Sant Cruz | 0,1 M | 5% swine | 1:50 | LSAB + System-HRP |
| sc-53546 | EDTA buffer pH8 | serum | over night | K 0690 | |
| PTEN | Cell Signaling | 0.01 M | 5% goat | 1:75 | Dako Real™EnVision™Detection System |
| 9188 s | Citrat buffer | serum | over night | K 5007 |
RT room temperature, FFP fresh frozen plasma.