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. 2014 Oct 9;9(10):e109755. doi: 10.1371/journal.pone.0109755

Figure 3. Detection of off-site targeting with PCR-RFLP assays in hamsters produced by DNA injection.

Figure 3

Genomic DNA isolated from toes clipped from the four targeted hamsters was used for detecting the off-site targeting with the PCR-RFLP assays. O1-O8: the eight potential off-site targets. Left panel shows PCR products before restriction enzyme digestion and the right panel shows PCR products after restriction enzyme digestion (note the band size changes after restriction enzyme digestions). *: multiple PCR bands due to non-specific DNA amplification (the bottom band is the correct PCR product).