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. 2014 Sep 25;15(9):468. doi: 10.1186/s13059-014-0468-1

Figure 3.

Figure 3

RNA-Seq analysis of N. coriiceps blood, brain, and skin tissues under cold and heat stress. (A) Genes significantly upregulated in response to heat stress or cold stress in whole blood (>two-fold). Bold characters represent genes shared between both stresses. Red characters represent genes related to the HSR. Expression value is on a log scale. Gene order was based on expression levels in whole blood. (B) qPCR results of HSP70, HSP40, Heat shock protein ssb1, and heat shock cognate protein 70 gene (HSC70). Beta-actin was used as a control. (C) Time course of HSP70 gene expression. The expression level of the HSP70 gene increased with increasing time exposure to heat or cold stress up to 48 h and decreased after 24 h of stress recovery.