Table 2.
Minimum inhibitory and cidal concentrations (μg/ml) of the extract, fractions and compounds from the stem of P pinnata against bacteria and fungi
| Microorganism | Test substance | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Extract b | PE f | EA f | AR f | 4 | 5 | 6 | 7 | RD | ||
| Bacteria | ||||||||||
| S. aureus | MIC | 781 | 1562 | 195 | 390 | 25 | 1.562 | 6.25 | 3.125 | 0.781 |
| MBC | 781 | 3125 | 390 | 1562 | 100 | 1.562 | 6.25 | 6.25 | 3.125 | |
| P. aeruginosa | MIC | 781 | 781 | 390 | 781 | 100 | 3.125 | 50 | 6.25 | 0.781 |
| MBC | 1562 | 3125 | 781 | 1562 | / | 6.25 | 100 | 12.5 | 1.562 | |
| K. pneumonia | MIC | 781 | 781 | 390 | 781 | 25 | 6.25 | 12.5 | 6.25 | 6.25 |
| MBC | 781 | 3125 | 781 | 1562 | 25 | 6.25 | 12.5 | 6.25 | 6.25 | |
| E. coli | MIC | 24 | 781 | 97 | 195 | 25 | 0.781 | 3.125 | 3.125 | 0.781 |
| MBC | 390 | 781 | 97 | 195 | 25 | 0.781 | 6.25 | 6.25 | 0.781 | |
| E. faecalis | MIC | 390 | 3125 | 781 | 1562 | / | 6.25 | 12.5 | 25 | 6.25 |
| MBC | 3125 | 6250 | 1562 | 1562 | / | 6.25 | 12.5 | 100 | 6.25 | |
| E. aeroginese | MIC | 390 | 390 | 97 | 195 | 25 | 0.781 | 6.25 | 12.5 | 1.562 |
| MBC | 781 | 781 | 195 | 390 | 50 | 1.562 | 12.5 | 25 | 1.562 | |
| P. smartii | MIC | 97 | 390 | 48.8 | 390 | 6.25 | 0.781 | 3.125 | 1.562 | 0.781 |
| MBC | 195 | 1562 | 195 | 781 | 12.5 | 0. 781 | 6.25 | 3.125 | 0.781 | |
| Yeasts | ||||||||||
| C. albicans | MIC | / | 1562 | 781 | 3125 | / | / | 3.125 | 3.125 | 3.125 |
| MFC | / | 3125 | 781 | 3125 | / | / | 6.25 | 6.25 | 3.125 | |
| C. guiliermondii | MIC | 12500 | 1562 | 195 | 3125 | / | / | 3.125 | 1.5625 | 6.25 |
| MFC | 12500 | 6250 | 195 | 6250 | / | / | 12.5 | 1.5625 | 12.5 | |
| C. neoformans | MIC | 3125 | 3125 | 195 | 3125 | / | / | 3.125 | 1.5625 | 1.562 |
| MFC | 6250 | 3125 | 390 | 3125 | / | / | 12.5 | 6.25 | 3.125 | |
| C. luciteniae | MIC | 3125 | 1562 | 97.7 | 781 | / | / | 3.125 | 3.125 | 3.125 |
| MFC | 6250 | 1562 | 195 | 1562 | / | / | 3.125 | 6.25 | 6.25 | |
| C. parapsilosis | MIC | 6250 | 1562 | 390 | 1562 | / | / | 6.25 | 6.25 | 3.125 |
| MFC | 12500 | 3125 | 390 | 1562 | / | / | 25 | 12.5 | 3.125 | |
| Dermatophytes | ||||||||||
| T. equinum | MIC | 12500 | 390 | 390 | 3125 | 50 | 50 | 25 | 50 | 6.25 |
| MFC | / | 1562 | 390 | 3125 | 100 | 100 | 25 | 50 | 12.5 | |
| M. audouinii | MIC | 12500 | 781 | 390 | 3125 | 25 | / | 6.25 | 12.5 | 6.25 |
| MFC | / | 1562 | 781 | / | 25 | / | 12.5 | 25 | 6.25 | |
| T. mentagrophytes | MIC | 12500 | 390 | 390 | 781 | 25 | 50 | 25 | 50 | 3.125 |
| MFC | / | 390 | 781 | 1562 | 50 | / | 25 | 50 | 6.25 | |
| M. gypseum | MIC | 12500 | 390 | 195 | 781 | 12.5 | 25 | 6.25 | 25 | 6.25 |
| MFC | 12500 | 1562 | 195 | 781 | 50 | 25 | 25 | 25 | 12.5 | |
| E. flocosum | MIC | 12500 | 390 | 195 | 781 | 25 | / | 12.5 | 50 | 3.125 |
| MFC | 12500 | 781 | 390 | 3125 | 25 | / | 25 | 100 | 6.25 | |
Extractb: stem extract; PEf: Petroleum ether fraction; EAf: ethylacetate fraction; ARf; aqueous residue fraction; RD: reference drug (Gentamycin, Nystatine and Griseofulvin for bacteria, yeasts and dermatophytes respectively); /: not active (at 12500 μg/ml for extract and fractions, and 100 μg/ml for compounds).