FIGURE 2.

LARGE GlcA-T reaction on the disaccharide acceptor. A, scheme depicting the reaction that LARGE GlcA-T activity catalyzes on the acceptor Xyl-α1,3-GlcA-β-MU. B and C, HPLC profile of the reaction product LARGEdTM generates from Xyl-α1,3-GlcA-β-MU and UDP-GlcA, as assessed by amide column (B) and LC-18 column (C). P, product. S, unreacted substrate. Dotted line, %B buffer. D, quadrupole time of flight (Q/TOF)-MS analysis of the product peak isolated in C. Star and diamond indicate Xyl and GlcA, respectively.