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GlcA-T activity of endogenous LARGE in mouse brain. A–D, LARGE GlcA-T activity on solubilized mouse brain membranes as a function of time (A), amount of protein (B), concentration of acceptor Xyl-α1,3-GlcA-β-MU (C), and donor UDP-GlcA (D). E, specific GlcA-T activity on brain membranes extracted from the WT (+/+, n = 3), heterozygous (+/−, n = 3), and homozygous Largemyd (−/−, n = 1) mice. Data are shown as relative activity (%).
