FIGURE 7.
Molecular interaction between α-DG and LARGE2. A, schematic representation of the α-DG-Fc fusion constructs used in the pull-down assay. The position of Thr-192 is indicated by an asterisk. B, representative data from pull-down of myc+His6-tagged LARGE2 with the α-DG-Fc fusion constructs, with or without the mucin and C-terminal domains, and with or without the T192M substitution (in the N-terminal domain). Immunoblotting with anti-myc antibody demonstrates that LARGE2 binds the N-terminal domain of α-DG, and that this binding is perturbed by the T192M mutation. Arrowhead indicates the position of myc+His6-tagged LARGE2.