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. 2014 Aug 20;289(41):28149–28159. doi: 10.1074/jbc.M114.593103

FIGURE 4.

FIGURE 4.

Effect of increasing channel open probability with PPi on Cd2+ sensitivity of TM6:TM12 double cysteine mutant channels. A, mean fractional current remaining following the addition of different concentrations of Cd2+ in M348C/S1141C channels in the presence of PKA and ATP (●) or following activation by PKA and ATP followed by treatment with 2 mm PPi to maximize channel open probability (○). B, mean Ki values estimated for the double cysteine mutant channels listed, either in the presence of PKA and ATP (black bars) or following treatment with PPi (gray bars). Asterisks indicate a significant difference in the presence of PPi (*, p < 0.05; **, p < 0.00005). Error bars indicate the means ± S.E. from 3–7 patches.