FIGURE 3.

36245-PHF inhibits multiple kinases in vitro. A, 36245-PHF inhibits PI3K kinase activity in vitro. Active kinase PI3K (p110α) and different doses of 36245-PHF or LY294002, a PI3K kinase inhibitor, were mixed with the substrate phosphatidylinositol and then incubated with a [γ-³²P]ATP mixture. The resulting radiolabeled spots were visualized by autoradiography. 36245-PHF also inhibits Aurora A (B) or Aurora B (C) kinase activity in a dose-dependent manner. The substrate histone H3 and active kinase Aurora A or Aurora B were mixed with different doses of 36245-PHF or hesperadin, a reported Aurora kinase inhibitor. Reactions were analyzed by Western blot. Data are represented as means ± S.D. from three independent experiments (*, p < 0.05 versus kinase only). DMSO, dimethyl sulfoxide.