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. 2014 Aug 19;289(41):28225–28236. doi: 10.1074/jbc.M114.568691

FIGURE 7.

FIGURE 7.

Effects of Nfic on Snail, Slug, E-cadherin, N-cadherin, vimentin, and F-actin in human dental pulp cells and odontoblastic MDPC-23 cells. A, Nfic, Snail, Slug, E-cadherin, and N-cadherin mRNA expression in Nfic+/+ (wild type) and Nfic−/− primary pulp cells analyzed by real-time PCR. B and C, expression of Nfic, Slug, E-cadherin, and vimentin mRNA (B) and protein (C) in MDPC-23 cells transfected with Nfic-expressing or Nfic siRNA constructs analyzed by real-time PCR and Western blotting, respectively. D, the transcriptional activity of Slug promoter was evaluated by luciferase assay in HEK293 cells transfected with Nfic-expressing or Nfic siRNA constructs. E, the transcriptional activity of E-cadherin promoter was evaluated by luciferase assay in HEK293 cells transfected with Nfic-expressing or Nfic siRNA constructs. Data are presented as the mean ± S.D. of triplicate experiments. An asterisk denotes values significantly different from the control (p < 0.01).