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. 2014 Aug 19;289(41):28225–28236. doi: 10.1074/jbc.M114.568691

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 9. — Effects of NFI family members on the expression of Klf4 and E-cadherin. A, expression of HA, Klf4, and E-cadherin protein was analyzed by Western blotting (left panel) and its quantification (right panel) in MDPC-23 cells transfected with HA-tagged Nfia-, Nfib-, Nfic-, or Nfix-expressing constructs. B and C, the transcriptional activity of the Klf4 or E-cadherin promoters was evaluated by luciferase assay in HEK293 cells transfected with Nfia-, Nfib-, Nfic-, or Nfix-expressing constructs. The data are presented as the mean ± S.D. of triplicate experiments. An asterisk denotes values significantly different from the control (p < 0.01).