Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Aug 28;289(41):28249–28259. doi: 10.1074/jbc.M114.589002

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

Author's Choice—Final version full access.

Creative Commons Attribution Unported License applies to Author Choice Articles

PMC Copyright notice

FIGURE 4. — ApoA-I epitope identification using digestion at lysine residues. A, chromatogram of LysC-derived peptides fractionation by reversed phase-high performance liquid chromatography. B and C, immunoreactivity of autoantibodies from positive patient sera (B) and negative patient sera (C) against peptides present in each collected fraction, and against the synthetic peptides A and B. D and E, chromatogram of the immunoreactive fraction 70 (D) and fraction 71 (E) by nano-liquid chromatography system. F and G, representative mass spectra of the peptide A (F) and peptide B (G) present in immunoreactive fractions by mass spectrometry (LTQ/Orbitrap). Relative error = (M_experimental − M_calculated)/M_calculated. Positive or negative sera: samples previously classified as containing high-titers or low titers of anti-apoA-I IgG, respectively.