TABLE 1.

Ligand binding affinity at WT AT1R and AT1R-β-arrestin2 fusion

The data are expressed as means ± S.E.

Ligand	pKi WT AT1R		pKi AT1R-β-arrestin2
	Isotonic (288 mOsm/kg)	Hypotonic (143 mOsm/kg)	Isotonic (288 mOsm/kg)	Hypotonic (143 mOsm/kg)
Angiotensin II	8.29 ± 0.03	8.36 ± 0.03	8.78 ± 0.02a	8.77 ± 0.03
TRV120023	6.74 ± 0.05	7.28 ± 0.03b	7.72 ± 0.03a	8.23 ± 0.02b
Telmisartan	8.68 ± 0.03	8.77 ± 0.03	8.66 ± 0.03	8.80 ± 0.03

^a p < 0.05, AT1R-β-arrestin2 fusion versus WT AT1R under isotonic conditions indicating functional coupling between the receptor and fused β-arrestin2 transducer (F test).

^b p < 0.05, hypotonic stretch (143 mOsm/kg) versus isotonic (288 mOsm/kg) buffer (F test).