FIGURE 1.

a, 800 MHz ¹H NMR spectrum of H. forskali fCS. b, conformation of fCS I, highlighting the GalNAc/fucose protons showing inter-residue NOEs (H-2/H-5 (purple), H-6/H-3 (yellow), H-6/H-4 (black), and H-2/H-6 (red)), as well as the distance (green) between fucose H-5 and the oxygen involved in the GalNAcβ4,6S(1→4)GlcAβ glycosidic bond in both fCS I and fCS II. c and d, expansions of an overlay of the two-dimensional ¹H-¹³C HSQC (red) and the two-dimensional ¹H-¹³C HSQC-NOESY (blue) spectra. The inter-residue NOE cross-peaks are circled. The methyl resonances of proteins are marked by an asterisk.