FIGURE 7.

Comparing stability and aggregation propensity between WT- and K35L-TTR. A, 1 μm WT-(filled circle) or K35L-TTR (hollow circle) were subjected to high hydrostatic pressure, and tryptophan fluorescence emission was collected to evaluate the extent of dissociation-denaturation. The experiments were performed at 25 °C and pH 7.5. B, acid-induced aggregation (3.5 μm, pH 4.4, 37 °C for 72 h) of WT- and K35L-TTR in the absence (solid bars) or presence of 35 μm heparin (hatched bars) as followed by absorbance at 330 nm. C–F, TEM images of the aggregates composed of the WT- or K35L-TTR grown in the absence (C and E, respectively) or in the presence of heparin (D and F, respectively). Bars, 1 μm. Error bars, S.E.