TABLE 3.
Kd values for StDicer-1 and StDicer-2 for duplex and hairpin RNAs
The reported equilibrium dissociation constants were calculated from the gel shifts in Fig. 4. The errors represent the standard fitting error. The concentration of the radiolabeled RNA used in these experiments was approximately 0.5–1 nm.
| 35-bp duplex | Hairpin RNA | |
|---|---|---|
| nm | nm | |
| StDicer-1 | 76 ± 14 | 33 ± 3 |
| StDicer-2 | 1800 ± 100 | 9600 ± 300 |
| StDicer-1 HD | >10,000 | 930 ± 80 |
| StDicer-2 HD | 3200 ± 400 | 6500 ± 2000 |
| ΔHel StDicer-2 | 900 ± 200 | 33 ± 3 |