FIGURE 10.

The RuvAB RFR product is a nascent strand duplex. A, the indicated restriction enzymes (1 unit of DpnI, 0.1 unit of MboI in lanes 5 and 10 and 0.01 unit in lane 9) were added to regression products formed by either RecG + RuvC or RuvAB in standard RFR reaction mixtures, and the incubations continued for 10 min at 37 °C. The DNA products were then analyzed by native gel electrophoresis. B, quantification of the fraction of CP2 or NDD that was resistant to digestion by the indicated restriction enzyme. The plot shows the mean and S.D. from two experiments. A representative gel is shown in A.