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Digestion of differentially methylated DNA by DpnI and MboI. Duplex DNA fragments (5.3 kbp) that were either methylated on both strands (A), hemimethylated (B), or unmethylated (C), prepared as described under “Experimental Procedures,” were treated with the indicated amounts of DpnI and MboI for 10 min at 37 °C in replication reaction buffer. The products of digestion were analyzed by native agarose gel electrophoresis.
