TABLE 1.
E. faecalis strains and plasmids used in this work
Cmr, Kanr, and Tetr indicate resistance to chloramphenicol, kanamycin, and tetracycline, respectively.
| Strain or plasmid | Relevant characteristics | Source/reference |
|---|---|---|
| OG1RF | Parental strain | 22 |
| ELF7 | katA::tet; Tetr | 23 |
| EMB4 | katA82 cydC::Tn; Tetr | 17 |
| pAM401 | E. coli/E. faecalis shuttle vector; Tetr, Cmr | 22 |
| pLUF15 | katA(His)6 in pAM401; Cmr | 6 |
| pLUMB29 | katA in pCR-Blunt II-TOPO; Kanr | This work |
| pLUMB31A | katA82 in pAM401; Cmr | This work |
| pLUMB38 | katA(Strep-tag II) in pAM401; Cmr | This work |