FIGURE 4.

The interaction between Fbxo45 and N-cadherin is sensitive to calcium. a, HT1376 cells were transfected with streptavidin/HA-tagged Fbxo45 and C-terminal V5-tagged N-cadherin deletion mutant (EC1–2). WCE were treated with 2 mm CaCl₂ or solvent control followed by IP with streptavidin resin. The Western blotting was performed with indicated antibodies. b, 293T cells were transfected with streptavidin/HA-tagged Fbxo45 (F45) or β-TrCP (Fbxw1), and WCE were treated with 2 mm CaCl₂ or solvent control followed by IP with streptavidin resin. Western blotting was performed with indicated antibodies. c, the experiment was performed as described in B. 2 mm EGTA was used wherever indicated.