TABLE 1.

Two-dimensional kinetic parameters of Lck on membranes

The result in Fig. 1E was compared with representative data for the two-dimensional (on liposome (24)) and three-dimensional (in solution phase (25)) kinetic parameters.

Membrane system	nH	kcat	Km	kcat/Km
		s−1	μm−2	×10−2s−1μm2
Supported bilayer	4.2	0.21	204	0.103
Liposome (24)	2.3	3.41	245	1.39
In solution (25)		0.0002–0.0098	2.3–21.7 (×10−5 m)	1.8–121.8 (m−1 s−1)