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The Nef·Hck32 complex forms a dimer of complexes in solution. Size-exclusion chromatography/multiangle light scattering elution profiles of the purified Hck32 protein (top), the Nef core (middle), and the Nef·Hck32 complex (bottom) are shown, with the refractive index trace in black (left y axes). The resulting weight-averaged molecular masses obtained from the elution peak of each protein or complex are plotted as the blue lines (right y axes).
