FIGURE 1.

Experimental strategy to explore the role of the S6-TRP box linker region of TRPM8. A, TRPM8 model depicting in red the S6-TRP box linker region. B, the schematic depicts a topological model of a TRPM8 subunit. Below the model are shown the amino acid sequences of the TRP domain proximal to S6 transmembrane segment of TRPM8 (blue) and TRPV1 (red). The protein regions in the S6-TRP box linker of TRPM8 replaced by the cognate of TRPV1 are indicated in red giving rise to chimeras ChA (Gly⁹⁸⁰-Ile⁹⁸⁵), ChB (Gly⁹⁸⁰-Asn⁹⁹⁰), and ChC (Gly⁹⁸⁰-Val⁹⁹³).