Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Ubiquitination-deficient TPP1 mutants are able to interact with TIN2, POT1, CTC1-STN1, and telomerase. Cells were co-transfected with the indicated constructs. 48 h post-transfection, extracts were prepared followed by anti-HA immunoprecipitation (IP) and Western blot analysis. TPP1(WT)-3xHA and ubiquitination-deficient mutants interact with TIN1–3xFLAG (A), POT1–3xFLAG (B), telomerase (C), and CTC1–3xFLAG/STN1-V5 (D). EV, empty vector.
