TABLE 2.
Kinetic parameters of AtCM isoforms
Reactions were performed as described under “Experimental Procedures.” All parameters are expressed as an average ± S.E. for an n = 3.
V/Et | Kmchorismate | kcat/Km | n (Hill coefficient) | |
---|---|---|---|---|
s−1 | μm | m−1 s−1 | ||
AtCM1 | 16.1 ± 0.4 | 550 ± 40 | 29,272 | |
AtCM2 | 38.7 ± 0.7 | 150 ± 9 | 258,000 | |
AtCM3 | 13.0 ± 1.2 | 1,100 ± 130 | 11,818 | 2.1 ± 0.2 |