TABLE 1.
NMR and refinement statistics for the Snu17p-Bud13p complex
NMR distance and dihedral restraints | |
---|---|
Distance restraints | |
Total NOE | 2607 |
Intra-residue | 636 |
Inter-residue | 1971 |
Sequential (|i – j| = 1) | 682 |
Medium range (|i – j| < 4) | 413 |
Long range (|i – j| > 5) | 747 |
Intermolecular | 129 |
Total dihedral angle restraints | 177 |
φ | 87 |
ψ | 90 |
Structure statistics | |
Violations (mean ± S.D.) | |
Distance restraints (Å) | 0.011 ± 0.00 |
Dihedral angle restraints (°) | 0.767 ± 0.07 |
Maximum dihedral angle violation (°) | 5.79 |
Maximum distance restraint violation (Å) | 0.34 |
Deviations from idealized geometry | |
Bond lengths (Å) | 0.011 ± 0.00 |
Bond angles (°) | 1.206 ± 0.03 |
Impropers (°) | 1.297 ± 0.06 |
Average pairwise root mean square deviationa (Å) | |
Heavy | 1.15 ± 0.17 |
Backbone | 0.55 ± 0.12 |
Ramachandran plot statistics (%) | |
Residues in most favored regions | 93.4 |
Residues in additionally allowed regions | 6.6 |
a Pairwise root mean square deviation was calculated among 20 refined structures for residues 30–109 of Snu17p and 222–239 of Bud13p.