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Vps4 ATPase activity and Ist1 inhibition are not disrupted by mutations in Vps4 α7 and α9. A, Coomassie staining of equivalent amounts of WT and mutant Vps4 proteins. B, titration (250 nm to 1.25 μm) of WT (black) and Vps4 mutants (M330K, blue; L407K, red; K411A, green) was performed in the presence of 4 mm ATP. Rates are indicated as ADP generated/Vps4/min. The concentrations yielding half-maximal activity (Km, app) and the apparent Vmax are indicated in the accompanying table.
