FIGURE 4.

Vps4 M330K and L407K partially disrupt in vitro association with GST-Vta1. GST or GST-Vta1 pulldown experiments were performed with purified WT and Vps4 mutants. Eluted material was analyzed by Coomassie staining and Western blotting for Vps4. WT and Vps4 mutants were isolated with GST-Vta1 more effectively than with GST alone. Vps4(WT) and Vps4(K411A) exhibited similar isolation with GST-Vta1, but isolation of Vps4(M330K) and Vps4(L407K) was reduced. The data presented are representative of experiments performed more than three times.