FIGURE 7.

Compensatory mutation of the VSE restores activation of Vps4(L407K). ATPase activity of 250 nm WT Vps4 (black) and Vps4(L407K) (red) in the presence of 8 μm VSL domain, WT Vta1, and Vta1(VSEΔ). The rates are indicated as ADP generated/Vps4/min. The activity of WT Vps4 in the presence of Vta1(VSEΔ) is reduced compared with activity with Vps4 and WT Vta1 (p value < 0.01; **). By contrast, the activity of Vps4(L407K) in the presence of Vta1(VSEΔ) is increased compared with activity with Vps4(L407K) with WT Vta1 (p value < 0.05; *).