FIGURE 6.

UV-induced H2AX phosphorylation in peripheral T lymphocytes in an NER-dependent manner. A, T lymphocytes isolated from thymus or lymph nodes of C57BL/6 mice were exposed to 20 J/m² of UV and incubated for 1 h or treated with 40 μg/ml of etoposide for 1 h. The cells were stained with anti-γH2AX antibody and propidium iodide and analyzed by flow cytometry. B, cell lysates were prepared from the isolated T lymphocytes and analyzed by Western blotting with the indicated antibodies. C and D, peripheral T lymphocytes from lymph nodes of wild-type or xpa knock-out mice were treated with UV or etoposide and analyzed for H2AX phosphorylation by flow cytometry (C) or Western blotting (D) with anti-γH2AX antibody. Ctrl, control; Etp, etoposide; pol, polymerase.