Figure 2. Intracellular ROS fluorescence and fluorescence rate under 40 Torr PO₂.

A: averaged fluorescence data recorded in a relative unit (RU). Data showed intracellular ROS levels from control, PO₂ cycling, ebselen (Eb), and PO₂ cycling + H₂O₂ treated diaphragm muscle (*significantly different from PO₂ cycling, P<0.05; ^#significantly different from Eb treatment, P<0.05; ^†significantly different from control, P<0.05). B: intracellular ROS burst represented by fluorescence rate. Data was recorded in a relative unit per min (RU/min) from control, PO₂ cycling, Eb, and PO₂ cycling + H₂O₂ (50 µM) treated diaphragm muscle under 40 Torr PO₂. Fluorescence data was recorded every 5 min (*significantly different from PO₂ cycling, P<0.05; ^#significantly different from Eb treatment, P<0.05; ^†significantly different from control, P<0.05).