Skip to main content
. 2014 Oct 9;9(10):e109981. doi: 10.1371/journal.pone.0109981

Figure 4. Telomere repeat amplification protocol (TRAP) analysis in U. maydis.

Figure 4

Telomerase activity in wild-type and mutant strains was determined. The absorbance data were used to construct a graphical representation of the telomerase activity for the sporidia of U. maydis strains (either wild-type or trt -). Tumor cells derived from the 521×520 cross and a plant control were included to evaluate and detect telomerase activity. The medians of the telomerase-positive control cells (HEK293) and the 521 wild-type strain were significantly different from the median of the treated negative controls (P<0.05); however, no significant differences were detected between the negative controls and the trt1-disrupted mutants. The samples heated to 85°C are indicated with Δ, and the RNase-treated samples are designated as RNase. Telomerase activity was also determined in tumors and maize leaves.