Table 4. Extrachromosomal trt1 complementation and growth rate restoration.

Strain	Transformant condition	Growth rates in min (hours)
521	Non-transformed	80 (1∶20 h)
	Transformed with pTrt1	285 (4∶45 h)
	Transformed with pNEBUC-GE77+	216 (3∶36 h)
W204	Non-transformed	815 (13∶35 h)
	Transformed with pTrt1 (T1)	122 (2∶02 h)
	Transformed with pNEBUC-GE77+	N.D.

After restoring the replicative potential by trt1 reintroduction, the growth rate was analyzed in the transformants with the same method used for the non-transformed controls. The strains analyzed are shown on the left, and the plasmids used to transform these strains are indicated at the top. In parentheses, minutes were changed to hours.