Figure 1. Schematic of the lentiviral vector used in this study.

The vector contains an effLuc expression cassette, expression of which is driven by the CMV promoter. An internal ribosomal entry site (IRES) and a mCherry cassette are downstream of luciferase. This vector contains the SIV promoter in the 5′ long-terminal repeat (LTR) for efficient virus production in the context of a Tat-free packaging system, and self-inactivating mutations in the 3′ LTR.