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. 2014 Oct 9;10(10):e1004423. doi: 10.1371/journal.ppat.1004423

Figure 3. Antigen translation efficiency regulates Ag-specific T cell trafficking and DC-T cell interactions.

Figure 3

Female C57BL/6 mice were adoptively transferred with CFSE+CD8+ OT-1 cells, followed by intramuscular immunization with adenoviral expression vectors Ad-E1-GArN-GFP or Ad-E1-GArM-GFP. DLNs were collected 2 days post-infection for frozen section. These were stained for nucleus, CD11c and Kb-SIINFEKL complex. (A) Representative merged images (×40) show the transferred CFSE+CD8+ OT-1 cells, CD11c-expressing cells and SIINFEKL epitope presenting cells. (B) Representative merged images at high magnification (×100) show the transferred CFSE+CD8+ OT-1 cells, CD11c-expressing cells and SIINFEKL epitope presenting cells. (C) Comparative analysis of the number of CFSE+CD8+ OT-1 cells in DLNs following infection with Ad-E1-GArN-GFP or Ad-E1-GArM-GFP. (D) Comparative analysis of the number of and SIINFEKL epitope presenting cells in DLNs following infection with Ad-E1-GArN-GFP or Ad-E1-GArM-GFP. (E) Representative images show the interactions between CD11c cells and SIINFEKL epitope presenting cells; CFSE+CD8+ OT-1 cells and SIINFEKL epitope presenting cells. (F) Comparative analysis of the interaction between CD11c cells with H-2Kb-SIIN+ cells. (G) Comparative analysis of the interaction between of H-2Kb-SIIN+ CD11c cells with CD8+ OT1 effector cells. (*p<0.05, Mann-Whitney).