Table 1.
List of primers used for qPCR.
| Gene | GenBank Accession Number | Forward Sequence (5'-3') | Reverse Sequence (5'-3') | Amplicon Size | Optimal Annealing Temperature Range (°C) |
|---|---|---|---|---|---|
| GAPDH | DQ403065 | 5'-ACGGCAAGTTC CACGGCACAGT-3' |
5'-GTTGGCGGGAT CTCGCTCCTGG-3' |
95 | 53.0–69.0 |
| Collagen Type II | AF201724 | 5'-CAAAGATGGCGA GACAGGTGCT-3' |
5'-GAAGTCCCTGGA AGCCAGATGGC-3' |
104 | 53.0–65.8 |
| Collagen Type I | AF201723 | 5'-GACGCACGGCCA AGAGGAGG-3' |
5'-CTGGCAGGGCAC GGGTTTCC-3' |
130 | 59.5–65.8 |
| Aggrecan | NM_001164652 | 5'-GCTTCCGAGGTGT CTCGGCG-3' |
5'-TCGTCTCCTCGC CCACAGGG-3' |
141 | 56.4–69.0 |