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. 2014 Oct 10;4:6580. doi: 10.1038/srep06580

Figure 6. CaMKII, ERK1/2 and CREB expression and phosphorylation in the brain of huApoE4 TR mice as compared to huApoE3 TR mice.

Figure 6

Immunoblotting of (a) CaMKII α-subunit (CaMKIIα), (c) ERK1/2 and (e) CREB expression and phosphorylation in E3 (white bar) and E4 (grey bar) TR mice at 12, 32 and 72 weeks of age. (A, C, E) The blot is a representative of five independent experiments. Blot images were cropped for comparison. Densitometric analysis of phosphorylated CaMKII-α (T286), ERK1(T202/Y204)/2(T185/Y187) and CREB (S133) was performed the NIH ImageJ software. The relative value for ApoE4 TR mice was normalized against age-matched ApoE3 TR mice. Each value represents the mean ± SEM for individual mouse brain sample. Phosphorylated CaMKIIα, ERK1/2 and CREB levels in E4 TR mice were significantly increased at 12 week but were significant reduced at 72 week as compared to E3 TR mice of similar age. (B) *p = 0.006, **p = 0.02 using Student's t-test. (D) *p = 0.03, **p = 0.02 using Student's t-test. (E) *p = 0.03, **p < 0.001 using Student's t-test.