Table 4. Rescue of homozygous nobo^KO larvae by oral sterol administration. The numbers of the first, second and third instar larvae of the nobo^KO homozygous larvae, which were fed standard cornmeal food with yeast pastes containing 0.5%(w/w) sterol/steroid supplement, are indicated. Homozygous nobo^KO larvae were obtained by maternal administration of cholesterol to homozygous nobo^KO embryos as shown in Table 3. Larval instars were scored at 72 hours AEL.

	Larval instar
Sterol/steroid	First	Second	Third
None	16	2	0
C	18	4	1
7dC	18	6	0
20E	1	4	19