Figure 2. Effects of isoforms and mutations on SaSMase C suppression of CFTR current.
(A) N-terminal sequences of three SaSMase C isoforms. (B) Percentage of SaSMase C suppression of CFTR current by individual isoforms at 1, 48 and 96 hr after final purification of SMase C (mean ± s.e.m, n = 5), where the final concentration was 0.4 ng/µl for isoform 1 and 40 ng/µl for isoforms 2 and 3. (C) Percentage of SaSMase C suppression of wild-type and mutant CFTR currents activated by expressing cRNA encoding the PKA catalytic subunit (mean ± s.e.m, n = 7–10).