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. 2014 Jul 16;4(3):662–677. doi: 10.3390/biom4030662

Figure 2.

Figure 2

Roles of chaperones UMP1, PAC1 and PAC3 during immunoproteasome biogenesis. (A) HeLa cells were treated with IFN-γ. After knockdown of β1, β2, and β5, α2i, β3, or UMP1 was further knocked down. Accumulated intermediates were detected by immunoblot using anti-α6 antibody; (B–D) The same panel as Figure 2A was immunoblotted with anti-β1i (B); anti-β2i (C); and anti-UMP1 (D) antibodies; (E–H) The same panel of Figure 1A was immunoblotted with anti-PAC1 (E); anti-PAC3 (F); anti-PA28α (G); and anti-PA28β (H) antibodies.