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. 2014 Aug 11;4(3):655–679. doi: 10.3390/metabo4030655

Figure 1.

Figure 1

Plasma KS and HS levels determined by Sandwich ELISA assay. Left panel (plasma KS): The KS standards for ELISA calibration and the anti-KS monoclonal antibody (5-D-4) were obtained from Seikagaku (Tokyo, Japan). The ELISA procedure was described previously [28]. The absorbance was measured at 450 nm using a microplate spectrophotometer reference to 650 nm. The KS concentration was determined by applying the absorbance of each sample to the calibration curve [28]. Right panel (Plasma HS): All reagents described for HS-ELISA were provided from Seikagaku Co (Tokyo, Japan). Monoclonal antibodies against HS were used. The ELISA method was essentially the same as described for KS assay. The absorbance was measured at 450 nm (reference absorbance 630 nm) using a microplate spectrophotometer [29].