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. 2014 Jun 6;592(Pt 17):3715–3726. doi: 10.1113/jphysiol.2014.274084

Figure 5. SUMO1 enhances α-cell exocytosis and prevents glucagon-like peptide-1 (GLP-1) receptor-mediated inhibition.

Figure 5

We measured exocytosis as increases in membrane capacitance in mouse α-cells, positively identified by glucagon immunostaining, in response to membrane depolarization. A, representative capacitance responses of mouse α-cells infected with green fluorescent protein (Ad-GFP; black traces) or SUMO1 (Ad-SUMO1; red traces). B, cumulative capacitance responses from mouse α-cells expressing Ad-GFP (open bars) and Ad-SUMO1 (black bars). Treatment with exendin 4 in the bath solution suppressed the exocytotic response, regardless of the presence of 0.1 mm or 1 mm cAMP in the patch pipette, but could not suppress the elevated responses observed in α-cells infected with Ad-SUMO1. *P < 0.05, **P < 0.01, ***P < 0.001 for comparisons with the respective control, or as indicated.