Table 3.
Post-translational modifications of the myosin heavy chain that are specific to mechanically ventilated rat at durations of 0.25–4 days, 5–8 days and 9–14 days, i.e. modifications not observed in controls
| Frequencya | ||||||
|---|---|---|---|---|---|---|
| Peptide sequence | Associated protein (Rattus norvegicus) | Amino acid modified | 0.25–4 days | 5–8 days | 9–14 days | |
| Deamidation | KKDFELNALNAR | Myosin 7 | N1088 | 3/11 | 1/5 | 3/6 |
| Deamidation | LQDAEEAVEAVNAK | Myosin 6 and myosin 7 | Q1398 | 3/11 | 1/5 | 2/6 |
| Deamidation | LTQESIMDLENDKQQLDER | Myosin 7 | N1071 | 1/11 | 0/5 | 2/6 |
| Deamidation | LTQESIMDLENDKQQLDER | Myosin 7 | Q1074 or Q1075 | 1/11 | 0/5 | 2/6 |
| Deamidation | NAESVKGMR | Myosin 6 and myosin 7 | N1833 | 6/11 | 2/5 | 1/6 |
| Deamidation | NDLQLQVQAEADGLADAEER | Myosin 4 | N889 or Q892 | 1/11 | 1/5 | 2/6 |
| Deamidation | QKYEESQSELESSQK | Myosin 6 and myosin 7 | Q1458 or Q1464 | 0/11 | 1/5 | 2/6 |
| Deamidation | RNNLLQAELEELR | Myosin 7 | N1677 or N1678 | 1/11 | 0/5 | 2/6 |
| Methylation | KKHADSAAELAEQIDNLQR | Myosin 3 | D1199 | 4/11 | 1/5 | 1/6 |
| Nitration | ILAEWK | Myosin 6 | W1457 | 2/11 | 1/5 | 1/6 |
| Oxidation | LAQESILDLENDKQQLDER | Myosin 3 | D1070 | 1/11 | 1/5 | 2/6 |
| Oxidation | NLTEEMAGLDENIVK | Myosin 4 | N992 or K995 | 1/11 | 2/5 | 3/6 |
a
Example: 3/11 means that this PTM was found in 3 animals out of 11 in total for that specific CMV group.