FIG. 5.

Channel elements of the HepaChip crucial for flow control during filling: (1) bifurcation of the inlet channel, (2) channel opening into assembly chamber, (3) merging of fluid streams at the chamber outlet, (4) channel constriction after each assembly chamber to ensure even filling of each of the eight chambers, and (5) merging of outlet channels into a single outlet channel. Yellow areas and lines resemble electrodes, connecting pads, and connecting lines intended for the application of high frequency electric fields to the cell chambers during dielectrophoretic assembly of cells. Scale bar 1 mm.