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The function of PIRB in platelet aggregation and secretion. (A-C) Aggregation of washed WT and PIRB-TM platelets was studied by aggregometery in response to agonists as indicated. (D-E) Washed WT and PIRB-TM platelets at a concentration of 3 × 107/mL were incubated with FITC-conjugated rat anti-mouse CD62P monoclonal antibody at 25°C for 20 minutes in the presence of (D) 0.5 μg/mL CRP or (E) 0.05 U/mL thrombin. The expression of CD62P was analyzed using a flow cytometer.
