Table 3.
Survival analysis of rrf-3 mutants treated with RNAi against DHHCs and PPTs
| RNAi strain | n | Mean (days) | Median (days) | 90% mortality (days) | Maximum (days) |
|---|---|---|---|---|---|
| L4440 | 208 | 16 | 16 | 25 | 31 |
| dhhc-1 | 79 | 16 | 16 | 24† | 25 |
| dhhc-11 | 47 | 12* | 10 | 18 | 21 |
| dhhc-14 | 99 | 19 | 19† | 22† | 31 |
| spe-10 | 79 | 18 | 19† | 24 | 29 |
| ppt-1 | 108 | 13 | 12† | 23 | 30 |
| ath-1 | 47 | 16 | 16 | 25 | 26 |
| RNAi strain(s) | n | Mean (days) | Median (days) | 90% mortality (days) | Maximum (days) |
| L4440 | 208 | 16 | 17 | 25 | 31 |
| dhhc-1 + dhhc-10 | 50 | 18 | 18 | 24 | 29 |
| dhhc-2 + dhhc-8 | 50 | 13* | 13 | 20† | 24 |
| dhhc-3 + dhhc-7 | 23 | 19 | 20 | 26 | 28 |
| dhhc-4 + spe-10 | 34 | 21* | 21† | 27 | 33 |
| dhhc-5 + dhhc-6 | 27 | 17 | 16 | 25 | 26 |
| dhhc-9 + dhhc-12 | 44 | 18 | 18 | 24 | 26 |
| dhhc-13 + dhhc-14 | 38 | 21 | 21† | 24 | 30 |
| ppt-1 + ath-1 | 143 | 14* | 14 | 23 | 30 |
Various measures of survival were extracted from lifespan experiments comparing hypersensitive rrf-3 animals treated with feeding RNAi empty vector L4440 control or against DHHC or PPT mutant strains (top). Mixtures of feeding RNAi bacteria were also used to assess simultaneous knockdown of related enzymes (bottom). Statistical tests were applied to values varying from the control using OASIS [73]. *p < 0.01 by the log-rank (Mantel-Cox) test. † p < 0.01 by Fisher’s exact test.r.