Fig. 4.

The effects of resveratrol on Ebox-Luc and Per1-Luc transcriptional activities. NIH3T3 cells expressing wild-type CLOCK, BMAL1, and either (A) Ebox-Luc or (B) Per1-Luc promoters were treated with increasing amounts of resveratrol (10 and 100 µM) to determine transcriptional activity. Luciferase activities in cell extracts were analyzed and normalized by cotransfected pRL-TK activity in each sample. To examine the effects of SIRTUIN1 (SIRT1) and resveratrol on the transcriptional activity of (C) Ebox-Luc and (D) Per1-Luc, SIRT1 was expressed with or without CLOCK and BMAL1 in NIH3T3 cells and incubated in resveratrol (10 and 100 µM). Values are expressed as mean±SEM in arbitrary units (AU), where the mean activity of the empty vector was set at 1 (n=3). ^aP<0.01 vs. vehicle-treated group.