Table 3.
Fluorophore–fluorophore and fluorophore–quencher pairs | Emission maxima (nm) | Fluorescence quantum yield of the fluorophore | Quenching factor | |
---|---|---|---|---|
1 | EtNBS-EtNBS | 681a | 0.2 | 4.5 |
2 | Cy5.5-Cy5.5 | 699a | 0.2 | 1.6 |
3 | Bodipy-FL-Bodipy-FL | 509b | 0.9 | 7.5 |
4 | Cy5.5-BHQ3 | 699a | 0.2 | 18.8 |
5 | Bodipy-FL-BHQ1 | 509b | 0.9 | 118 |
Spectra were recorded in methanol.
Spectra were recorded in 20% DMSO in PBS solution.